It really depends on several factors, I don't know where to start. First off, search engine optimization might be a bit hard - but aside from that, from my expereinece, it doesn't matter much. You might use a little bit of traffic, but not really anything significant. Also depends on what you want for the subdomain to be. For a forum I highly recommend using a subdomain - it just sorts your site out better. But for most other things, an extention should be fine. But two or three subdomains won't kill ya'.
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